adenosine adora2a antibody  (Alomone Labs)

Journal: Pharmacological research

Article Title: Blockade of endothelial adenosine receptor 2A suppresses atherosclerosis in vivo through inhibiting CREB-ALK5-mediated endothelial to mesenchymal transition

doi: 10.1016/j.phrs.2024.107156

Figure Lengend Snippet: Loss of endothelial Adora2a suppressed the formation of atherosclerotic lesions.

Article Snippet: The aortas were blocked with 10% goat serum (#50062Z, Thermo Fisher, Waltham, MA) for 1 hour at room temperature and incubated with primary antibodies against ADORA2A (1 μg/ml, Santa Cruz, sc-32261), CD31 (1 μg/ml, Dianova, DIA310), ACTA2 (1 μg/ml, Santa Cruz, sc-56499) or ALK5 (1 μg/ml, Santa Cruz, sc-518086) overnight at 4°C.

Techniques:

Journal: Cells

Article Title: Sevoflurane Dampens Acute Pulmonary Inflammation via the Adenosine Receptor A2B and Heme Oxygenase-1.

doi: 10.3390/cells11071094

Figure Lengend Snippet: Figure 1. Time-dependency of the anti-inflammatory effects of sevoflurane on PMN migration and the influence of sevoflurane on the expression of adenosine receptors. Sevoflurane was applied one hour before (pre) LPS inhalation, immediately after LPS (sim), or three hours (post) after LPS stimulation. (A) Polymorphonuclear neutrophil (PMN) sequestration into the intravascular space (IV), interstitium (IS), and bronchoalveolar lavage (BAL) was assessed in wild-type animals by flow cytometry (n = 6–8). (B) The gene expression of the adenosine receptors A2A (Adora2a) and A2B (Adora2b) in the pulmonary

Article Snippet: To tackle the Adora2a and Adora2b on PMNs, we used FITC-conjugated Adora2a (sc-32261; Santa Cruz Biotechnologies; USA) and PE-conjugated Adora2b (120319; Novus Biotechnologies; Centennial, CO, USA) specific antibodies.

Techniques: Migration, Expressing, Flow Cytometry, Gene Expression